[From Sanger to genome sequencing - an overview of DNA sequencing technologies].

🤖 Plain-English Summary

There is no technique that would make a greater contribution to the development of genetics, molecular biology and medicine than DNA sequencing. We present their short history, principles of operation, technical possibilities, applications and limitations.

🔑 Key Findings

• For many years, the method based on enzymatic DNA synthesis developed by Frederic Sanger was the gold standard in this area.
• At the end of the 20th century, there was a dynamic development of next-generation sequencing (NGS) technologies, which ended the era of single gene analysis and initiated the era of genome sequencing.
• Despite fierce competition, one NGS technology has practically completely dominated the global market.

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