Protocol for a human placental explant model to study acute responses to pathogens.

🤖 Plain-English Summary

Here, we present a protocol for studying infections caused by pathogens associated with intrauterine complications-Listeria monocytogenes, Plasmodium falciparum, and Toxoplasma gondii-using an ex vivo placental explant model. This protocol allows short-term placental infection studies.

🔑 Key Findings

• We describe procedures for pathogen culture, preparation of placental explants using an air-liquid interface system, and infection assays.
• We detail procedures for tissue dissociation and CD45+ cell enrichment.
• This protocol allows short-term placental infection studies.

🔗 Related Resources