Murine brain tumor microenvironment immunophenotyping using mass cytometry.

🤖 Plain-English Summary

Here, we present a mass cytometry protocol optimized to examine the phenotype of immune cells within the mouse glioma microenvironment, using a Sleeping Beauty transposon-mediated mouse glioma model. This protocol can be applied to any brain tumor-harboring mouse model.

🔑 Key Findings

• We describe antibody conjugation and titrations for analysis of immune cells.
• We then detail mouse brain tumor tissue collection and processing, staining, followed by data acquisition, analysis, and gating strategy.
• This protocol can be applied to any brain tumor-harboring mouse model.

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