Inference of CRISPR Edits from Sanger Trace Data

🤖 Plain-English Summary

Efficient and precise genome editing requires a fast, quantitative, and inexpensive assay to assess genotype following editing. The ICE tool is free to use and open source, and offers several improvements over current analysis tools, such as batch analysis and support for a variety of editing conditions.

🔑 Key Findings

• Here, we present ICE (Inference of CRISPR Edits), which enables robust analysis of CRISPR edits using Sanger data.
• ICE proposes potential outcomes for editing with guide RNAs, and then determines which are supported by the data via regression.
• The ICE algorithm is robust and reproducible, and it can be used to analyze CRISPR experiments within days after transfection.

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