Implementation and validation of single-cell genomics experiments in neuroscience.

🤖 Plain-English Summary

Single-cell or single-nucleus transcriptomics is a powerful tool for identifying cell types and cell states. The choice of validation depends on numerous factors.

🔑 Key Findings

• However, hypotheses derived from these assays, including gene expression information, require validation, and their functional relevance needs to be established.
• The choice of validation depends on numerous factors.
• Here, we present types of orthogonal and functional validation experiment to strengthen preliminary findings obtained using single-cell and single-nucleus transcriptomics as well as the challenges and limitations of these approaches.

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