Assessing mRNA translation in mouse adult microglia and bone-marrow-derived macrophages.

🤖 Plain-English Summary

Protein synthesis, or mRNA translation, is the biological process through which genetic information stored in messenger RNAs is encoded into proteins. We then detail their quantification by flow cytometry or with a fluorescent plate reader.

🔑 Key Findings

• Here, we present an optimized protocol for assessing the translation rate in mouse adult microglia and cultured bone-marrow-derived macrophages.
• We describe steps for isolating cells, treating them with a puromycin-analog probe, and fluorescently labeling the puromycylated-polypeptide chains.
• We then detail their quantification by flow cytometry or with a fluorescent plate reader.

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