Uncovering spatially resolved functional genomics with CRISPR screen sequencing.

Spatial omics has advanced our understanding of tissue-level biology, yet tools to systematically link gene functional perturbations to spatial phenotypes and signaling pathways remain limited. We also demonstrated the model of the transcription factor-chemokine receptor axis coupling cell states with chemotaxis.

⚡ This is an original paraphrased summary — not copied from the abstract. Full paper available at the source link below.

• 1 To address this, we developed spatial CRISPR screen sequencing (SPAC-seq), a high-throughput spatial CRISPR screen platform, and TARDIS (target prioritization toolkit for perturbation data in spatial omics), a statistical spatial perturbation analysis toolkit.
• 2 Using SPAC-seq and TARDIS, we linked gene perturbations to spatial phenotypes and pathways, uncovering how Icam1 loss in tumor cells promotes metastasis via immune suppression and macrophage polarization.
• 3 In CD8 T cells, we revealed Cd44's role in regulating spatial phenotypes by interacting with Spp1 on macrophages.

Understanding this could lead to better treatments, improved diagnostics, or a deeper grasp of how the human body works — benefiting patient care globally.

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