Protocol for a human placental explant model to study acute responses to pathogens.

Here, we present a protocol for studying infections caused by pathogens associated with intrauterine complications-Listeria monocytogenes, Plasmodium falciparum, and Toxoplasma gondii-using an ex vivo placental explant model. This protocol allows short-term placental infection studies.

⚡ This is an original paraphrased summary — not copied from the abstract. Full paper available at the source link below.

• 1 We describe procedures for pathogen culture, preparation of placental explants using an air-liquid interface system, and infection assays.
• 2 We detail procedures for tissue dissociation and CD45+ cell enrichment.
• 3 This protocol allows short-term placental infection studies.

Understanding this could lead to better treatments, improved diagnostics, or a deeper grasp of how the human body works — benefiting patient care globally.

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