Murine brain tumor microenvironment immunophenotyping using mass cytometry.

Here, we present a mass cytometry protocol optimized to examine the phenotype of immune cells within the mouse glioma microenvironment, using a Sleeping Beauty transposon-mediated mouse glioma model. This protocol can be applied to any brain tumor-harboring mouse model.

⚡ This is an original paraphrased summary — not copied from the abstract. Full paper available at the source link below.

• 1 We describe antibody conjugation and titrations for analysis of immune cells.
• 2 We then detail mouse brain tumor tissue collection and processing, staining, followed by data acquisition, analysis, and gating strategy.
• 3 This protocol can be applied to any brain tumor-harboring mouse model.

Understanding this could lead to better treatments, improved diagnostics, or a deeper grasp of how the human body works — benefiting patient care globally.

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